Tissue Engineering of the Liver
نویسندگان
چکیده
1. Background . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 419 1.1. Liver Disease and Cell-Based Therapies . . . . . . . . . . . . . . . . . . . . . . . . 419 1.2. Cell Sources for Liver Cell-Based Therapies . . . . . . . . . . . . . . . . . . . . 419 1.3. Approaches for the In Vitro Stabilization of Primary Hepatocytes . 420 1.4. Regulation of the Hepatocyte Microenvironment Toward the Development of Engineered Liver Tissue . . . . . . . . . . . . . . . . . . . . . . . 422 2. Preparation of Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 424 2.1. Krebs-Ringer Buffer (KRB) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 424 2.2. KRB with EDTA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 424 2.3. Collagenase, 0.055% (w/v) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 424 2.4. Coating Buffer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 424 2.5. OPD Substrate Buffer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 424 2.6. Complete Hepatocyte Culture Medium (CHCM) . . . . . . . . . . . . . . . 425 2.7. Blocking Buffer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 425 2.8. Washing Buffer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 425 2.9. Piranha Cleaning Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 425 2.10. Hepatocyte Culture Medium, Serum Free (HCM/SF) . . . . . . . . . . . . 425 2.11. Fibroblast Culture Medium (FCM) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 425
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